臺灣博碩士論文加值系統

根據衛生福利部統計民國106年臺灣十大死因中，慢性肝炎及肝硬化排名第十，在十大癌症死亡率中，肝和肝內膽管癌亦高居第二名，由此可知肝病對國人健康的嚴重性。而羅布麻Apocyuum venetum L.，具有抗過敏、降血壓、抗癌、延緩衰老等保健功能。故在本研究中即針對羅布麻酒水萃取物 ( AE )，先以人類肝癌細胞HepG2細胞株進行抑制率之分析；另依衛生福利部所頒布健康食品之護肝功效評估方法，進行四氯化碳 ( Carbon tetrachloride, CCl4 ) 誘發ICR小鼠急性肝炎之實驗模式，經由血清生化檢測、肝臟抗氧化活性分析以及病理組織切片的觀察，以評估AE之護肝功效，實驗中並以Silymarin ( 200.0 mg/kg ) 作為陽性對照藥物；最後進行保肝之成分分析。實驗結果顯示：在HepG2細胞抑制率分析中，以500 µg/mL AE對人類肝癌細胞HepG2細胞作用24小時有明顯的抑制作用，抑制率為41.6%。在急性肝炎之血清生化檢測中，其ALT值在Control組; CCl4誘發組; Silymarin組及AE之低、中、高劑量組分別為：46.5 ± 9.8; 932.8 ± 104.0; 675.5 ± 82.8; 799.8 ± 90.8; 1099.8 ± 9.0; 453.3 ± 109.4 U/L。由上述結果得知，實驗小鼠受到CCl4誘發急性肝炎，使血清中ALT值明顯高於Control組 ( p<0.05 )，但當小鼠投予AE高劑量後，血清中的ALT值明顯低於CCl4誘發組 ( p<0.05 )。而根據抗氧化實驗結果顯示，老鼠經CCl4誘發肝炎後，會導致肝臟MDA含量增加，而GSH、SOD及CAT之活性則下降，但反觀餵食AE的樣品組，則發現小鼠肝臟的MDA含量 ( p<0.05 ) 明顯降低，並增強GSH、SOD及CAT之活性。另由H&E染色後的肝組織病理切片發現，投予AE組的小鼠可降低CCl4所造成肝細胞的發炎反應、脂肪變性。另外在保肝成分的分析結果得知: AE含有槲皮素、金絲桃苷及異槲皮苷等三種護肝成分，其中以異槲皮苷的含量最高。綜合上述實驗結果得知，AE對CCl4所誘發急性肝炎之實驗動物，確實具有顯著的護肝功效，其作用機轉值得後續更進一步之研究。

According to the Ministry of Health and Welfare statistics, among the top ten causes of death in Taiwan in the Republic of China in 106, chronic hepatitis and cirrhosis ranked tenth. Among the top ten cancer death rates, liver and intrahepatic cholangiocarcinoma also ranked second, which indicates that liver disease is against Chinese people. The severity of health. Apocyuum venetum L. has anti-allergic, blood pressure lowering, anti-cancer, anti-aging and other health functions. Therefore, in this study, we aimed to analyze the inhibition rate of human hepatocarcinoma cell HepG2 cell line for apocynum venetum water extract ( AE ), and conduct tetrachlorination according to the evaluation method of liver health efficacy of health foods issued by the Ministry of Health and Welfare. Carbon tetrachloride ( CCl4 ) induces acute hepatitis in ICR mice. The serum biochemical test, liver antioxidant activity analysis and pathological tissue section observation are used to evaluate the liver function of AE. The experiment is performed with Silymarin ( 200.0 mg/kg ) as a positive control drug; finally, component analysis of liver protection was performed. The results showed that in the inhibition rate of HepG2 cells, the effect of 500 μg/mL AE on human hepatoma HepG2 cells was inhibited for 24 hours, and the inhibition rate was 41.6%. In the serum biochemical test of acute hepatitis, the ALT value in the Control group; CCl4 induced group; Silymarin group and AE low, medium and high dose groups were: 46.5 ± 9.8; 932.8 ± 104.0; 675.5 ± 82.8; 799.8 ± 90.8 1099.8 ± 9.0; 453.3 ± 109.4 U/L. From the above results, it was found that the experimental mice were induced by CCl4 to induce acute hepatitis, and the ALT value in the serum was significantly higher than that in the Control group ( p<0.05 ). However, when the mice were administered a high dose of AE, the ALT value in the serum was significantly lower than that. CCl4 induced group ( p<0.05 ). According to the results of antioxidant experiments, mice induced by CCl4 induced an increase in MDA content in the liver, while the activities of GSH, SOD and CAT decreased, but in the sample group fed AE, the MDA content in the liver of the mice was found ( p<0.05 ) was significantly reduced and enhanced the activities of GSH, SOD and CAT. In addition, hepatic histopathological sections stained by H&E showed that mice administered to the AE group reduced the inflammatory response and steatosis of hepatocytes caused by CCl4. In addition, the results of the analysis of liver-protecting components revealed that: AE contains three kinds of liver-protecting components such as quercetin, hyperoside and isoquercitrin, among which the content of isoquercitrin is the highest. Based on the above experimental results, it is known that AE has significant liver-protecting effects on experimental animals induced by CCl4, and its mechanism is worthy of further research.

摘要 I
致謝 IV
圖目錄 VII
表目錄 X
縮寫表 XI
第一章 前言 1
第二章 文獻回顧 3
第一節 肝臟簡介 3
第二節 四氯化碳 5
第三節 水飛薊素 6
第四節 羅布麻之介紹 7
第五節 羅布麻保肝成分 9
第三章 材料與方法 16
第一節 研究架構 16
第二節 研究目的 17
第三節 研究材料 17
第四節 羅布麻樣品之製備 21
第五節 羅布麻抑癌作用之細胞實驗 21
第六節 羅布麻之動物實驗 23
第七節 血清生化指數的檢測 24
第八節 肝臟病理組織切片與H&E染色 24
第九節 肝臟抗氧化之測定 24
第十節 羅布麻之成分分析 31
第十一節 統計分析 37
第四章 結果 38
第一節 羅布麻酒水萃取物對人類肝癌細胞HepG2細胞抑制率之分析 38
第二節 羅布麻酒水萃取物之急性肝炎試驗 40
第三節 羅布麻酒水萃取物之成分分析試驗 71
第五章 討論 82
第一節 羅布麻酒水萃取物對急性肝炎試驗動物之血清生化值的影響 82
第二節 羅布麻酒水萃取物對急性肝炎試驗動物之肝臟抗氧化的影響 83
第三節 羅布麻酒水萃取物對急性肝炎試驗動物之肝臟組織病理學觀察 85
第四節 羅布麻酒水萃取物成分分析之影響 85
第六章 結論 87
第七章 參考文獻 88

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