臺灣博碩士論文加值系統

本研究中，我們提出了一個新型比色探針 (TNT@MB)，藉由磷酸鹽與二氧化鈦奈米管 (TNT) 之間強結合力來檢測磷酸鹽。二氧化鈦奈米管藉由二氧化鈦奈米粒子水熱合成。二氧化鈦奈米管平均長度為200 ± 50 奈米，平均寬度為12 ± 5 奈米。二氧化鈦奈米管的表面電位藉由動態光散射儀 (DLS) 檢測。結果得知二氧化鈦奈米管的零點電荷 (pHpzc) 為4。所以比色探針藉由TNT在酸性環境下吸附甲基藍製備，且吸附行為可透過靜電作用解釋。 TNT@MB的最佳合成條件為0.05g TNT與1μmole甲基藍在pH 2下反應90分鐘。
TNT與TNT@MB的性質藉由紫外可見光漫反射光譜儀、穿透式電子顯微鏡、FT-IR光譜儀及X射線電子能譜進行分析。TNT@MB檢測磷酸鹽是藉由紫外可見光光譜進行研究。檢測磷酸鹽的線性範圍為1- 40μM，偵測極限為0.59μM。我們在相同條件下檢測典型陰離子和金屬離子存在下的吸收強度變化，結果表明TNT @ MB系統中的吸收只有很小的干擾。通過對湖水樣品的檢測，研究了該方法的回收率實驗，回收率為102.5％~103.6％，相對標準偏差為5.6％（n = 3）。此外，該方法還用於檢測ATP，ADP和AMP。通過在鹼性溶液中除去TNT表面的甲基藍和磷酸鹽，然後TNT再次吸附甲基藍，重複步驟進行再生循環研究。三次循環後，再生的TNT仍然可以保持幾乎100％的甲基藍吸附效率，這表明TNT @ MB具有優異的可回收性。這些結果表明，此比色探針具有環保，廉價，簡單且易於觀察的優點。

In the proposed study, we report a novel colorimetric probe (TNT@MB) to detect phosphate based on a strong binding ability of phosphate with titanium dioxide nanotube (TNT). The titanium dioxide nanotube (TNT) was synthesized from titanium dioxide nanoparticles (P25) by hydrothermal treatment. The obtained TNT had an average length of 200 ± 50 nm and average width of 12 ± 50 nm. The zeta-potential of TNT was investigated by dynamic light-scattering (DLS). The result showed that the pH of point of zero charge (pHpzc) of TNT was 4. Therefore, the colorimetric probe (TNT@MB) was prepared by adsorbing methyl blue with TNT in acidic condition and the adsorption behavior could be interpreted via the electrostatic interaction. The optimal synthesis condition of TNT@MB was reacted by 0.05g TNT with 1μmole methyl blue at pH 2 for 90 min.
The properties of TNT and TNT@MB were characterized by UV-vis diffuse reflection spectrum, transmission electron microscopy (TEM), FT-IR spectrum and X-ray electron spectroscopy (XPS). The sensing behaviors of TNT@MB toward phosphate were investigated by UV-visible spectroscopy. The linear range and the detection limit of the adsorption method based on TNT@MB for the detection of phosphate were 1- 40μM and 0.59μM, respectively. We examine the absorption intensity changes in the presence of typical anions and metal ions under the same conditions and the results suggested that there was only small interference in the absorption of the TNT@MB system. The recoveries experiment of the proposed method was studied by detecting the lake water samples and the recoveries were from 102.5% to 103.6% while relative standard deviation bellowed 5.6% (n=3). Moreover, this method also used to detect ATP, ADP and AMP. The regeneration cycle study was performed by removing the methyl blue and phosphate of the TNT surface in an alkaline solution and then methyl blue was adsorbed again by TNT. Interestingly, the regenerated TNT can still maintain almost 100% of methyl blue adsorption efficiency even after three cycles, indicating the TNT@MB had excellent recyclability. These results indicate that the colorimetric probe had the advantages of being environmentally friendly, inexpensive, simple, and easy to observe.

口試委員會審定書 ii
致謝..................................................................................................................................iv
中文摘要 v
Abstract vi
目 錄 vii
圖 目 錄 ix
表 目 錄 xi
Chapter 1 緒論 1
1.1 前言 1
1.1.1 磷酸鹽的測定 1
1.1.2 材料的選擇 4
1.1.3 研究目的 6
Chapter 2 實驗方法 7
2.1 化學試劑 7
2.2 儀器分析與鑑定 7
2.3 TNT與TNT@MB之合成 8
2.4 磷酸鹽之檢測方法 8
2.5 重複利用性 9
2.6 湖水中檢測磷酸鹽 9
2.7 ATP, ADP和AMP的檢測 9
Chapter 3 結果與討論 10
3.1 TNT與TNT@MB的鑑定 10
3.2 合成TNT@MB的條件優化 17
3.3 TNT@MB檢測磷酸鹽 21
3.4 TNT@MB檢測ATP、ADP及AMP 31
Chapter 4 結論 33
參考文獻 34

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