臺灣博碩士論文加值系統

　　急性運動可對身體功能引起許多暫時性的影響，例如內分泌及心血管系統等。然而，對於腦功能的影響就目前為止尚未清楚。大腦衍生神經滋養因子(BDNF)，為神經滋養因子家族的一員，透過其受體－旋轉肌凝蛋白相關蛋白質激酶B(TrkB)扮演著調控神經可塑性的重要角色。過去的研究指出，BDNF及其受體TrkB的表現受到神經元活性所調控，而在我們過去的實驗中也指出，單一次的運動可以短暫的讓大鼠腦中海馬迴(hippocampus)中的BDNF蛋白表現量上升。在本實驗中，我們主要探討的是急性運動是否可以調控不同腦區中BDNF及其受體TrkB的表現。我們使用三個月大的小鼠(品系為C57BL/6，簡稱B6)做不同的運動方式：運動訓練四週、單一次的急性中度或劇烈運動，並在運動後不同時間點犠牲，取出其海馬迴、小腦(cerebellum)及皮質額葉(frontal cortex)來作蛋白質或mRNA的分析。我們的結果顯示，急性中度運動後，海馬迴中TrkB mRNA的表現量立即有顯著的增加，而蛋白質的表現則是在二到四小時後有明顯的上升。然而BDNF並未有顯著的改變。小腦則是在運動後立即觀察到全長型的TrkB蛋白(Full-length TrkB,TrkB.FL)有顯著性的上升，然而在皮質額葉則是都沒有變化。而訓練四週小鼠的海馬迴BDNF在最後一次運動後四小時則是有顯著上升,full-length TrkB則顯示了運動的慢性效果，然而這些蛋白在小腦及皮質額葉則都沒變化。這些結果已初步排除壓力或糖皮質激素的影響。相反的，急性劇烈運動後一小時則有降低TrkB蛋白表現的趨勢，但無顯著差異。這些結果指出，不同運動模式及腦區對於B6小鼠腦中BDNF及TrkB的調控有不同的影響，而這些改變對於生理上的意義仍有待進一步證實。

　　Acute exercise induces transient changes in our body function, such as endocrine and cardiovascular systems. Whether it influences the brain function is still unclear. Brain-derived neurotrophic factor (BDNF), a member of neurotrophin family, plays an important role in neuroplasticity via its receptor – tropomyosin-related kinase B (TrkB). The expression of BDNF and its receptor TrkB is activity-dependent. It has been shown that a single bout of exercise acutely increases rat hippocampal BDNF protein level in our previous study. In this study, we want to further clarify if acute exercise can modulate BDNF and TrkB in different brain areas. To address this issue, male C57BL/6 mice (3-month-old) were subjected to different exercise protocols. i.e. 4 weeks moderate exercise training, a single bout of moderate or severe exercise, and were sacrificed at different time course after exercise. Hippocampus, cerebellum and frontal cortex were collected for the measurement of mRNA or protein expression. The results of mice experienced acute moderate exercise showed that hippocampal TrkB mRNA expression was increased immediately after exercise, and its protein expression was elevated 2 and 4 hours later. However, BDNF was not changed. Cerebellar full-length TrkB protein was increased immediately after exercise. There was no significant change of these two proteins in the frontal cortex. The exercise-trained mice increased their hippocampal BDNF 4 hours after the last run when compared to the control group, and TrkB expression was higher than control even two days after exercise training; however, there was no change of these two proteins in cerebellum or frontal cortex. These results may be independent of stress, because of the comparable levels of serum corticosterone among all studied groups except the 0-hr one. In contrast, acute severe exercise tended to decrease hippocampal expression of TrkB 1 hour after exercise. These results indicate a differential regulation of BDNF and TrkB by either different exercise regimes or in different brain areas after exercise in the brain of C57BL/6 mouse. The physiological significance of these changes needs to be further clarified.

Abstract in Chinese…………………………………………………………………I
Abstract……………………………………………………………………………II
Acknowledgments…………………………………………………………………III
Table ofcontents…………………………………………………………………IV
List of Table………………………………………………………………………V
List of Figures……………………………………………………………………VI
List of Appendix………………………………………………………………VII
Introduction………………………………………………………………………1
Materials and Methods……………………………………………………………7
Results……………………………………………………………………………21
Discussion………………………………………………………………………25
Conclusion………………………………………………………………………31
References………………………………………………………………………32
Table………………………………………………………………………………39
Figures……………………………………………………………………………41
Appendix…………………………………………………………………………62
About the Author ………………………………………………………………75

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