臺灣博碩士論文加值系統

從羅氏鹽膚木(Rhus javanice L. var. roxburghiana)之果實部位的萃取物進行抗氧化的測試，分別對DPPH自由基、ABTS自由基和xanthine oxidase，結果顯示具有相當好的抗氧化活性。並且從羅氏鹽膚木的果實部位，分離得到kaempferol (5)，這是首次在果實部位得到這種化合物。另外，也將本科室之前從羅氏鹽膚木的莖部所分離得到的四個化合物taxifolin (1) 2,3-cis-3,4-trans-7,4’-dihydroxyflavan-3β,4β-diol (2) 、fustin (3)、7,3’,4’-trihydroxy-flavone (4)分別對DPPH自由基、ABTS自由基和xanthine oxidase進行抗氧化試驗。結果發現化合物(1)、(2)和(3)對DPPH自由基的清除能力有相當不錯的活性。而taxifolin (1)對xanthine oxidase有不錯的抑制能力，是未來可以發展的方向。
從chemotaxonomy方面可知果實的活性成分可能亦屬於化合物(1)-(4)之類似物。

The extract obtained from the fruit of Rhus javanice L. var. roxburghiana was performed several biological studies associated with free radical, including DPPH,ABTS, and xanthine oxidase inhibitory assays. Among these studies, the extract exhibited significantly inhibitory effect on production of free radicals. In this study, kaempferol (5) was first isolated from fruit of Rhus javanice L. var. roxburghiana. On the other way, four compounds, taxifolin (1) ,2,3-cis-3,4- trans-7,4’-dihydroxy- flavan-3β,4β-diol (2) , fustin (3), 7,3’,4’-trihydroxy-flavone (4), previously isolated, from the stem of this plant were performed DPPH, ABTS, and xanthine oxidase assays. Compounds (1), (2) and (3) showed significantly inhibitory activity. As these result shown, taxifolin (1) was found as a potent xanthine oxidase inhibitor.
Based on chemotaxonomic theory, the above four compounds (1)-(4) may be also appears in the extract of fruit of this plant.

目錄------------------------------------------------------I
圖表目錄------------------------------------------------III
中文摘要--------------------------------------------------V
英文摘要-------------------------------------------------VI
第一章
緒論------------------------------------------------------1
第一節 研究動機與目的---------------------------------1
第二節 研究背景---------------------------------------3
第二章
羅氏鹽膚木(Rhus javanica var. roxburghiana)化學成分研究---5
第一節 植物型態與分佈---------------------------------5
第二節 文獻回顧---------------------------------------7
第三節 研究材料與方法--------------------------------24
第四節 kaempferol(5)之結構解析與證明-----------------28
第三章
抗氧化試驗與結構活性關係---------------------------------29
第一節 前言-----------------------------------------29
第二節 DPPH自由基清除效力測定-----------------------36
第三節 ABTS自由基清除效力測定-----------------------40
第四節 Xanthine Oxidase活性測定---------------------45
第五節 化合物結構與活性關係-------------------------51
第四章
結論-----------------------------------------------------54
第五章
實驗儀器與材料-------------------------------------------56
第一節 儀器----------------------------------------56
第二節 材料----------------------------------------58
第六章
參考文獻-------------------------------------------------60

圖表目錄
圖2-1 羅氏鹽膚木的果實--------------------------------6
圖3-1 ABTS的結構和與抗氧化劑RH的反應-----------------32
圖3-2 DPPH的結構和與抗氧化劑RH的反應-----------------32
圖3-3 羅氏鹽膚木之果實部位的甲醇萃取物和維他命E的DPPH
測定-------------------------------------------39
圖3-4 羅氏鹽膚木之莖部所分離得到的四個化合物與維他命E
的DPPH測定-------------------------------------39
圖3-5 羅氏鹽膚木之果實部位的甲醇萃取物和維他命E的ABTS
測定-------------------------------------------44
圖3-6 羅氏鹽膚木之莖部所分離得到的四個化合物與維他命E
的ABTS測定-------------------------------------44
圖3-7 xanthine oxidase的試驗-------------------------45
圖3-8 羅氏鹽膚木之果實部位的甲醇萃取物進行xanthine
oxidase抑制活性測定的結果----------------------49
圖3-9 羅氏鹽膚木之莖部所分離得到的四個化合物與
allopurinol的xanthine oxidase抑制活性測定------49
表3-1 羅氏鹽膚木莖部所分離得到的四個化合物分別對DPPH、
ABTS和xanthine oxidase測定其IC50以及SEM--------50
圖3-10 xanthine enol form和黃酮類作比較---------------53

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