臺灣博碩士論文加值系統

中 文 摘 要 由多孔菌科(Polyporaceae)之靈芝屬(
Ganoderma)的松杉靈芝(G. tsugae Murr.)，分離出三個新化合物，分別
是：Tsugaric acid A (1)、Tsugaric acid B (5) 及3a-Acetoxy-5a-
lanosta-8,24-dien-21-oic acid glucose ester (Tsugaric acid A
glucose ester) (6) 和四種已知化合物， 它們是：Ergosta-7,22-
dien-3b-ol (2) 、3b-Hydroxy-5a-lanosta-8,24-dien-21-oic acid (3)
、3-Oxo-5a-lanosta-8,24-dien-21-oic acid (4)及 2b,3a,9a-
Trihydroxy-5a-ergosta-7,22-diene (7)。為了更進一步証明1和3的結構
，分別進行皂化反應及酯化反應，得到3a-Hydroxy-5a-lanosta-8,24-
dien-21-oic acid (1a) 和3b-Acetoxy-5a-lanosta-8,24-dien-21-oic
acid (3a)。將其中的1，1a，3，3a和5等羊毛甾烷類固醇衍生物進行人類
癌細胞毒殺的活性篩選，結果顯示，Tsugaric acid A (1)對人類膀胱癌
細胞 (T-24 cells)有顯著的抑制活性，又3b- Hydroxy-5a-
lanosta-8,24-dien-21-oic acid (3)與3b-Acetoxy-5a-lanosta-8,24-
dien-21-oic acid (3a)也分別對人類子宮頸癌細胞(HT-3 和CaSki
cells)有明顯的抑制活性。而其他的化合物也都具有邊際活性 (marginal
effects)。

Abstract Three new compounds isolated from Ganoderma tsugae
Murr. (Polyporaceae) including : Tsugaric acid A (1) , Tsugaric
acid B (5) , 3a-Acetoxy-5a-lanosta-8,24-dien-21-oic acid glucose
ester (Tsugaric acid A glucose ester) (6) , and four known
compounds including : Ergosta-7,22-dien-3b-ol (2) , 3b-Hydroxy-5
a-lanosta-8,24-dien-21-oic acid (3) , 3-Oxo-5a-lanosta-8,24-
dien-21-oic acid (4) , and 2b,3a,9a-Trihydroxy-5a-ergosta-7,22-
diene (7) were investigated. In order to elucidate the
structures of 1 and 3 , saponification and acetylation were
carried out to obtain 3a-Hydroxy-5a-lanosta-8,24-dien-21-oic
acid (1a) and 3b-Acetoxy-5a-lanosta-8,24-dien-21-oic acid (3a) ,
respectively. Lanostane-type triterpenoid derivatives 1 , 1a , 3
, 3a and 5 were screened for cytotoxic activities against PLC/
PRF/5 , HT-3 , SiHa , CaSki, T-24 , and 212 tumor cells in
vitro. The results indicated that 1 showed significant
cytotoxicity against T-24 cells in vitro, 3 and 3a also showed
significant cytotoxicity against HT-3 , and CaSki cells in vitro
. The other compounds exhibited marginal effects against several
human tumor cell lines.