臺灣博碩士論文加值系統

長期飲用紅酒被認為對心血管有益，文獻研究也指出紅酒中的多酚類具有抗氧化作用，可能是保護心血管的主要作用機轉。紅酒內含二十幾種多酚，白藜蘆醇是其中的一種，它也是中藥虎杖主要的有效成份之一，長久以來處方用於降血脂、抗發炎及心血管疾病。解剖學研究發現動脈粥樣硬化局部性的好發於某些特定的區域，推測可能與血液的機械特性有關。本文試以血液流變學的觀點來探討白藜蘆醇對心血管保護作用的機轉。天竺鼠餵食有或無添加白藜蘆醇的高膽固醇飼料一個月。採取高膽固醇血液樣本及餐後高三酸甘油血液樣本，分析生理參數及血液流變學參數，結果發現白藜蘆醇可以明顯降低紅血球丙二醛，但無法降低天竺鼠血中的膽固醇及三酸甘油濃度，對高膽固醇所引發的紅血球聚集度下降及高三酸甘油所引發的紅血球聚集度上升無明顯影響，也無法改善高膽固醇所引發的紅血球變形度下降。血管內皮細胞體外試驗發現白藜蘆醇能中和自由基，降低乳酸去氫脢釋出，有保護血管內皮細胞的作用。高膽固醇血症會改變紅血球的機械特性，也會引發自由基造成紅血球細胞膜脂質的過氧化，白藜蘆醇無法改善紅血球的機械特性，對保護心血管的作用機轉可能是來自於捕捉自由基，降低脂質過氧化，保護血管內皮細胞膜的完整性。

Long-term moderate consumption of red wine is thought to have positive effect on arteries. Research literature suggested that the antioxidation of polyphenols in red wine might possess major cardiovascular protective mechanism. Red wine contains more than twenty kinds of polyphenols including resvertrol. Resveratrol is one of the active ingredients in Huzhang, a Chinese herb, which has long been used to lower blood lipids and fight inflammation as well as cardiovascular disease. Anatomic studies found that Atherosclerosis predisposed on some particular areas and this phenomenon may be associated with hemodynamics. This paper approaches the cardiovascular protective mechanism of resveratrol in terms of hemorheology. Guinea pigs were fed with high cholesterol feeds with or without resveratrol for a month. Blood samples of high cholesterol and after meal high triglyceride were taken. Physiological parameters and hemorheological parameters were analyzed. Research found that resveratrol could effectively lower malondialdehyde but could not lower the cholesterol and triglyceride. It had no significant effect upon RBC aggregation induced by hypercholesretolemia and hypertriglyceridemia. It could not improve deformability of erythrocyte caused by hypercholesretolemia. Endothelium in vitro assays found that resveratrol could neutralize free radicals, lower the liberation of lactate dehydrogenase and protect endothelium. Hypercholesterolemia can change some mechanical properties of RBC and produce free radicals, which can result in lipid peroxidation on plasma membrance. Resveratrol cannot improve the mechanical properties of erythrocyte. The cardiovascular protective mechanism of resveratrol may come from scavenging free radicals to lower lipid peroxidation, and therefore protect the membrane integrity of endothelium.

致 謝…………………………………………………………………I
中文摘要（Abstract in Chinese）……………………………………II
英文摘要（Abstract in English）……………………………………III
目 錄（Contents）…………………………………………………IV
圖表目錄（Lists of Figures and Tables）…………………………VI

第壹章 緒論（Introduction）…………………………………………1
第貳章 實驗材料與方法（Materials and Methods）
一、實驗動物…………………………………………………………24
二、檢體採集…………………………………………………………25
三、檢體前處理………………………………………………………26
四、紅血球細胞膜丙二醛之測定法…………………………………26
五、紅血球脆性試驗…………………………………………………28
六、纖維蛋白原（Fibrinogen）定量………………………………29
七、全套血球計數……………………………………………………31
八、生化試驗…………………………………………………………32
九、血漿黏度及血液黏度測定………………………………………32
十、紅血球變形度（erythrocyte deformability）測定…………33
十一、紅血球聚集度（erythrocyte aggregation）測定……………34
十二、乳酸去氫脢釋出試驗……………………………………………35
十三、自由基捕捉試驗…………………………………………………37
十四、統計分析…………………………………………………………39
第參章 實驗結果（Results）
一、實驗動物抽血前的外觀及抽血後死亡率………………………40
二、高膽固醇飼料對生理參數及血液流變參數之影響……………40
三、高三酸甘油對生理參數及血液流變參數之影響………………41
四、白藜蘆醇對高膽固醇餵食動物之影響…………………………41
五、白藜蘆醇對高三酸甘油血症動物之影響………………………42
六、白藜蘆醇可降低乳酸去氫脢（LDH）釋出………………………42
七、白藜蘆醇可中和白血球氧爆所產生的自由基…………………43

第肆章 討論（Discussion）……………………………………………44

第伍章 參考文獻（Reference）…………………………………………52

第陸章 圖表（Figures and Tables）…………………………………59

附錄（Appendix）………………………………………………………72

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