臺灣博碩士論文加值系統

在雄性禿髮形成的原因中，5alpha-還原酶扮演著關鍵的角色。目前在治療雄性禿髮的方法主要以柔沛(finasteride)與落建(minoxidil)兩種藥物為主。研究顯示，柔沛可透過抑制第二型5alpha-還原酶的作用，促進男性雄性禿的毛髮生長；落建透過尚未清楚的作用機轉，促進毛髮生長。
某些種類的大豆異黃酮已經證明可以抑制第二型5alpha-還原酶，因此在本研究中，以動物實驗方式，評估含大豆異黃酮的大豆抽出物對於改善雄性禿髮的效果。研究分兩部分進行，第一部份以雄性禿老鼠(B6CBAF1 mouse)的動物模式，藉以探討活性型及非活性型大豆異黃酮素萃取物，對第二型5alpha-還原酶抑制能力的評估，及雄性禿老鼠抑制掉毛及促進毛髮生長情形。第二部分以C57BL/6 mouse alopecia model，評估非特異性掉髮的毛髮增生。第一部份研究結果顯示，活性型大豆異黃酮素萃取物在0.5%與1%的濃度，都有抑制雄性禿掉毛及促進毛髮生長情形，組織學切片檢查，也符合臨床及病理表現。而非活性型大豆異黃酮素萃取物，則無法抑制雄性禿掉毛。第二部分研究結果顯示活性型大豆異黃酮素萃取物也有促進非雄性禿脫毛的毛髮增生，但作用機轉是否與大豆異黃酮素多樣的藥理活性有關尚不清楚。這兩項研究結果提供了雄性禿髮往後相關的研究方向。

5alpha-reductase plays a crucial role in the pathogenesis of androgenetic alopecia. At present, there are two main drugs, finasteride and minoxidil, designed to treat androgenetic alopecia. Study showed that the inhibitor of 5alpha-reductase type 2, such as finasteride, promoted hair growth in male pattern alopecia. Also, minoxidil can promote hair growth by uncertain mechanism.
Some kinds of soy isoflavones have been proved to have abilities on the inhibition of 5alpha-reductase type 2. Therefore, we evaluate the effect of soy isoflavone extracts on the inhibition of androgenetic alopecia in animal model. There are two parts in our studies. The first part is androgenetic alopecia animal model (B6CBAF1 mouse) to evaluate the inhibition of 5alpha-reductase type 2 and promotion in hair growth when soy isoflavone extracts (aglycone type and glycone type) are used. The second part is C57BL/6 mouse alopecia model to evaluate hair growth in non-specific hair loss. The first part show that either 0.5% or 1% soy isoflavone extracts (aglycone type) can inhibit hair loss and promote hair regrowth in AGA mice. Histopathologic findings from skin of mice are compatible with clinical pictures. However, neither 0.5% nor 1% soy isoflavone extracts (glycone type) can inhibit hair loss of androgenetic alopecia of mice. The second show that soy isoflavone extracts (aglycone type) also promote hair growth on non-specific hair loss. It is not yet clear whether the mechanism of action is related to various pharmacological activities of soy isoflavone. The results of these two studies provide a direction in the future research of androgenetic alopecia.

中文摘要------------------------------------------------------------------------Ⅰ
英文摘要------------------------------------------------------------------------Ⅲ
誌謝------------------------------------------------------------------------------Ⅴ
目錄------------------------------------------------------------------------------Ⅵ
圖目錄---------------------------------------------------------------------------Ⅷ
表目錄---------------------------------------------------------------------------Ⅻ
第一章 緒論---------------------------------------------------------------------1
1-1研究背景與動機------------------------------------------------------------1
1-2文獻探討---------------------------------------------------------------------3
1-2-1 頭髮的解剖生理---------------------------------------------------------3
1-2-2 雄性素在皮膚的代謝，及對毛髮生長的影響---------------------9
1-2-3 雄性禿的致病機轉與治療---------------------------------------------15
1-2-4 類黃酮素的篩選及生物活性------------------------------------------26
1-2-5 與掉髮有關的老鼠動物試驗模式------------------------------------32
1-2-5-1 與雄性禿實驗有關的動物模式------------------------------------32
1-2-5-2 其他非雄性禿脫毛的毛髮增生實驗------------------------------34
1-3 研究目的--------------------------------------------------------------------35
第二章 材料與方法------------------------------------------------------------36
2-1使用的材料------------------------------------------------------------------36
2-2實驗方法---------------------------------------------------------------------38
(1)利用B6CBAF1 mouse model 評估大豆異黃酮素萃取物對雄性禿抑制作用及促進毛髮生長-------------------------------------------------38
(2)利用C57BL6 mouse model 評估大豆異黃酮素萃取物對其他非特異性掉毛的毛髮增生實驗------------------------------------------------40
第三章 結果與討論------------------------------------------------------------43
3-1利用B6CBAF1 mouse model 評估大豆異黃酮素萃取物對雄性禿抑制作用及促進毛髮生長-----------------------------------------------43
3-2利用C57BL6 mouse model 評估大豆異黃酮素萃取物對其他非特異性掉毛的毛髮增生實驗------------------------------------------------62
第四章 結論---------------------------------------------------------------------69
第五章 未來的展望------------------------------------------------------------71
參考文獻--------------------------------------------------------------------------73

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