臺灣博碩士論文加值系統

之前的研究顯示，採檢體積未達建議容量，及抗凝劑或保存劑的種類之不同，其對於血液酒精濃度有可能造成影響。本實驗以外加酒精的方式於空白血液中，探討不同的採檢體積對於血液酒精濃度在分析上可能造成的誤差，結果顯示採檢體積越少，越有可能低估血液酒精濃度達20 %。
臨床上採檢血液酒精濃度的標準作業方法，係採用含氟化鈉 (NaF) 抗凝劑的灰頭管採血以避免細菌發酵產生酒精，並在保持真空的狀態下儲存（室溫可穩定2天、於2-8℃可穩定14天），但是在某些較為特殊的情況下並沒有氟化鈉 (NaF) 抗凝劑的檢體可供檢驗。之前的文獻認為不同的鹽類造成酒精因為鹽析效應以致造成誤差，可以用內標準品作為代償。本實驗以一般的臨床檢驗流程，在進入氣相層析質譜儀(GC-MS) 分析前才加入內標準品，探討鹽類可能造成的影響，結果顯示使用含有Heparin及EDTA的採血管其酒精濃度較接近外加的酒精濃度 (50 mg/dL)，而使用含有NaF的採血管相較於EDTA採血管則可能被低估達10 %。

According to the former reasearches, insufficient volume of blood sample and different types of collecting tubes may affect the analytical results of alcohol concentration. Our study showed that insufficient volume of blood sample leads to excess air space on the top of the tube where ethanol vapors and makes underestimation of blood alcohol concentration.
Sodium fluoride (NaF) collecting tubes can suppress glycolysis and ethanol formation is widely used for analysis of the blood alcohol concentration. In our study, comparison with NaF collecting tubes, blood alcohol concentration analysis using Ethylenediaminetetraacetic acid (EDTA) and Heparing tubes are more close to the expected value.

第一章 緒論9
1. 前言9
2. 文獻回顧10
2.1 保存溫度對酒精檢測的影響10
2.2 採血管的不同對於酒精檢測的影響10
2.3 頂空法與GC-MS的應用12
3. 研究動機13
第二章　材料與方法15
1. 實驗材料15
1.1 血液樣本15
1.2 標準品試劑15
1.3 採血管15
2. 實驗儀器設備16
3. 實驗方法16
3.1 實驗分組16
3.2 分析方法18
第三章　實驗結果19
1. 方法確效19
1.1 檢量線19
1.2 Intraday Repeatability19
1.3 Interday Reproducibility19
1.4 LOD/LOQ20
2. 檢體分析20
2.1 第一組為研究採血管在分析前是否經過混合對血液酒精濃度的影響20
2.2 第二組為研究採檢體積對血液酒精濃度的影響21
2.3 第三組為研究不同採血管對血液酒精濃度的影響23
第四章 討論25
1. 檢體混合與否對血液酒精濃度的影響25
2. 採檢體積對血液酒精濃度的影響26
3. 不同採血管對血液酒精濃度的影響27
第五章　結論與建議29
參考文獻32

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