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研究生:張維倫
研究生(外文):Wei-Lun Chang
論文名稱:Pseudomonas stutzeri 和Pseudomonas alcaligenes 處理養殖廢水總氨-氮及亞硝酸-氮之影響
論文名稱(外文):The Effect of Pseudomonas stutzeri and Pseudomonas alcaligenes on the total ammonia-N and nitrite-N in aquaculture wastewater
指導教授:冉繁華冉繁華引用關係
指導教授(外文):Fan-Hua Nan
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:59
中文關鍵詞:施氏假單胞菌產鹼假單胞菌水質益生菌
外文關鍵詞:Pseudomonas stutzeriPseudomonas alcaligeneswater qualityProbiotic
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  • 被引用被引用:1
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本篇研究先建立Pseudomonas stutzeri和Pseudomonas alcaligenes兩株菌的生長曲線和最適的培養條件包括鹽度、pH及溫度,同時進行兩種菌對養殖生物的安全性評估,再一步評估對養殖廢水及飼料廢水處理之效果。本篇著重討論P. stutzeri和P. alcaligenes不同添加濃度和型式下,處理養殖廢水和飼料廢水中比較氨-氮及亞硝酸-氮的效果。
結果顯示,P. stutzeri培養在NB培養基中起始濃度為106 CFU/ml,鹽度5 ‰、pH 7、溫度37 ℃及150 rpm震盪,在第10小時為生長曲線之高峰,菌量為1.17×109 CFU/ml;P. alcaligenes培養在NB培養基中起始濃度為106 CFU/ml,鹽度5 ‰、pH 7、溫度37 ℃及150 rpm震盪,在40小時為生長曲線之高峰,菌量為5.22×108 CFU/ml。
P. stutzeri 最佳的生長環境,鹽度5 ‰、pH 7~ 8及溫度37 ℃;Pseudomonas alcaligenes 最佳的生長環境,鹽度15 ‰、pH 7及溫度37 ℃。
腹腔注射0.5 ml 106及108 CFU/ml 的P. stutzeri及P. alcaligenes菌液於紅色吳郭魚體內,觀察七天且每濃度進行評估三次,活存率均為100%,且在血液、肝臟、膽、頭腎及腸均無檢測到本實驗使用菌株。
結果顯示P. stutzeri 處理養殖廢水中氨-氮去除率與時間無相關性,去除率106 CFU/ml組有明顯大於104及105 CFU/ml處理組。兩株菌混合處理養殖廢水中氨-氮去除率與時間無相關性,去除率106 CFU/ml組有明顯大於104及105 CFU/ml處理組。P. alcaligenes 處理養殖廢水及飼料廢水中氨-氮及亞硝酸-氮均與控制組無顯著差異 (p>0.05)。
P. stutzeri處理不同鹽度飼料廢水中氨-氮去除率與時間有相關性,且去除率在鹽度0 ‰組明顯大於15 ‰及35 ‰組;兩株菌混合不同鹽度飼料廢水中氨-氮去除率與時間有正相關性,鹽度0 ‰組明顯大於15 ‰。P. stutzer及兩株菌混合處理不同鹽度飼料廢水亞硝酸-氮時,只有在第5天P. stutzer處理鹽度0 ‰組與控制組有顯著差異 (p&;lt;0.05)。P. stutzeri處理鹽度0 ‰組,在氨-氮濃度趨近於0的同時,飼料廢水的亞硝酸-氮開始有下降趨勢。P. alcaligenes 處理飼料氨-氮及亞硝酸-氮廢水處理效果鹽度0‰組及15‰組與控制組無顯著差異 (p>0.05)。

謝辭...................i
中文摘要...............ii
英文摘要..............iii
目錄..................iv
表目錄.................v
圖目錄................vii
前言....................1
文獻整理.................2
材料方法.................7
結果...................14
討論...................18
結論...................21
參考文獻................22
附錄...................58

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