臺灣博碩士論文加值系統

中文摘要
本研究目的是設計以幾丁聚醣和三聚磷酸鈉作為藥物輸送系統，並應用於觀察Vitamin E之釋放。
三聚磷酸鈉-幾丁聚醣微膠囊以不同濃度的幾丁聚醣和三聚磷酸鈉溶液以及不同p H值的三聚磷酸鈉溶液藉著乳化內相膠凝法來製備。而當降低溫度時則會有相轉移，即為溶膠-凝膠(sol-gel)過程。
三聚磷酸鈉-幾丁聚醣微膠囊之Vitamin E釋放性質是以仿效人體體液和親脂系統來做釋放測試。
結果指出Vitamin E在較高的幾丁聚醣溶液濃度和三聚磷酸鈉溶液中會有較緩慢的釋放。而在較低的pH值三聚磷酸溶液中，Vitamin E從三聚磷酸鈉-幾丁聚醣微膠囊中釋放比在較高pH值的三聚磷酸鈉溶液中較為緩慢。

Abstract
The purpose of this study is to design the drug delivery system by using chitosan and tripolyphosphate(TPP), and apply the devices to observe the release of Vitamin E.
TPP-chitosan microcapsules were prepared with various concentrations of chitosan and TPP and various pH of TPP solutions by the method of emulsification/internal gelation. The process shows phase change when temperature decreases that is the sol-gel.
The release behaviors of Vitamin E from TPP-chitosan microcapsules were investigated with phosphate-buffered saline (PBS) and hydrophobic system.
The results indicate that the release of Vitamin E is slower both with higher concentrations of chitosan and of TPP solutions. And the TPP solutions of low pH value have slower Vitamin E release from TPP-chitosan microcapsules than those of high pH value.

總目錄
中文摘要 Ⅰ
英文摘要 Ⅱ
總目錄 Ⅲ
目錄 Ⅳ
表索引 Ⅶ
圖索引 Ⅷ
目錄
第一章 緒論
1.1 前言.........................................1
1.2微膠囊介紹....................................2
1.2.1微膠囊...................................2
1.2.2微膠囊的特點.............................3
1.2.3微膠囊包覆技術...........................3
1.3 sol-gel 系統（溶膠與凝膠系統）...............6
1.4藥物控制釋放技術簡介..........................7
1.5幾丁質與幾丁聚醣的介紹.......................10
1.5.1 幾丁質與幾丁聚醣的構造.................10
1.5.2 幾丁質與幾丁聚醣分布...................13
1.5.3 幾丁質與幾丁聚醣之溶解性質.............13
1.5.4 幾丁質類物質之物理測定.................17
1.6 維他命 E ( Vitamin E).......................20

第二章 實驗原理
2.1 本實驗製備三聚磷酸鈉-幾丁聚醣微膠囊形成原理..............................................21
2.2流變學( rheology )...........................22
2-3 粒徑分析....................................26
2.4掃描式電子顯微鏡(Scanning Electron Microscopy，SEM)............................................28
2.5 Zeta電位(Zeta potential)量測................29

第三章 實驗
3.1 藥品........................................31
3.2 儀器........................................33
3.3 幾丁聚醣流變學性質測定......................34
3.4 微膠囊的製備................................35
3.5 幾丁聚醣濃度的影響..........................35
3.6 交聯劑三聚磷酸鈉濃度的影響..................36
3.7 三聚磷酸鈉水溶液在不同pH值之影響............36
3.8 微膠囊粒徑大小測定..........................36
3.9 微膠囊掃描式電子顯微鏡(SEM)觀測.............37
3.10 三聚磷酸鈉-幾丁聚醣微膠囊Zeta電位的測定..............................................37
3.11 Vitamin E檢量線的製作......................37
3.12 微膠囊內Vitamin E含量測定..................40
3.13 微膠囊釋放特性分析.........................40
3.13.1 仿效人體體液系統........................40
3.12.2 仿效人體親脂系統........................40

第四章 結果與討論
4.1 幾丁聚醣流變學性質..........................42
4.2 幾丁聚醣濃度對三聚磷酸鈉-幾丁聚醣微膠囊粒徑、包覆量的影響....................................46
4.3 三聚磷酸鈉濃度對三聚磷酸鈉-幾丁聚醣微膠囊粒徑、包覆量的影響................................49
4.4三聚磷酸鈉水溶液pH值之影響...................52
4.5 電子顯微鏡 (SEM) 分析微膠囊外觀.............55
4.6 pH值對於三聚磷酸鈉-幾丁聚醣微膠囊Zeta電位(Zeta Potential)的討論..........................61
4.7 釋放的結果探討..............................66
第五章 結論.....................................78
第六章 參考文獻.................................79
第七章 附錄.....................................84

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