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研究生:李政億
研究生(外文):Cheng-Yi Lee
論文名稱:諾麗果汁對 Streptozotocin- Nicotinamide 誘導糖尿病雄性大鼠生殖功能之影響
論文名稱(外文):Effects of Noni Juice on Male Reproductive Function of Rats of Streptozotocin-Nicotinamide Induced Diabetes
指導教授:龔瑞林龔瑞林引用關係
指導教授(外文):Zwe-Ling Kong
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:88
中文關鍵詞:諾麗果糖尿病不孕症男性生殖氧化壓力
外文關鍵詞:Morinda citrifoliaNoniDiabetesMale reproduction
相關次數:
  • 被引用被引用:4
  • 點閱點閱:831
  • 評分評分:
  • 下載下載:44
  • 收藏至我的研究室書目清單書目收藏:0
糖尿病造成男性不孕,其主要因素是精子受到氧化壓力傷害,導致精子受損,以及生殖內分泌調節軸受到影響,使內分泌紊亂、性腺功能發生異常。本研究目的為探討諾麗果汁對於 streptozotocin (STZ)-nicotinamide (NA) 誘發之糖尿病雄性大白鼠生殖功能的影響。以細胞實驗檢測樣品之劑量安全性並評估諾麗果汁中所含之有效成分 asperulosidic acid (AA) 及 deacetylasperulosidic acid (DAA) 對 advanced glycation end products (AGEs) 誘導 TM3、TM4 及 LC-540 細胞株生成自由基之抑制效果。AA 及 DAA 在濃度 20 g/ml 對細胞不具有毒性,且能夠顯著地抑制 superoxide 生成。動物實驗以 60 隻雄性 Sprague-Dawley 大白鼠為實驗對象,利用腹腔注射 nicotinamide (230 mg/kg) 及 STZ (65 mg/kg) 誘導大白鼠罹患糖尿病。實驗分組為:(1) 正常組 (2) 糖尿病組 (3) 糖尿病並給予抗糖尿病藥物 Rosiglitazone (RSG) 組 (4) 糖尿病給予諾麗果汁一倍劑量組 (1.24 g/kg bw) (5) 糖尿病給予諾麗果汁兩倍劑量組 (2.48 g/kg bw) (6) 糖尿病給予諾麗果汁五倍劑量組 (6.2 g/kg bw),為期四週。結果顯示,餵食諾麗果汁無法明顯改善高血糖及胰島素阻抗情形,但卻能改善血脂異常情形並能夠促使雄性激素荷爾蒙分泌,改善生精作用。諾麗果汁能夠降低睪丸中 receptor for advanced glycation end products (RAGE) 表現量,提高抗氧化酵素活性並降低脂質過氧化程度,使糖尿病大鼠精子數量及品質獲得改善。本研究証實,補充大溪地諾麗果汁能夠有效改善糖尿病大鼠之生殖氧化壓力,具有開發為機能性保健食品之潛力。
Hypogonadism and oxidative stress occurs commonly in men with diabetes associated male infertility. This study aims to investigate the possible ameliorating effect of Noni juice dietary supplement on streptozotocin (STZ)-nicotinamide (NA) induced male reproductive alterations in Sprague-Dawley rats. In vitro, 20 g/ml of asperulosidic acid (AA) and deacetylasperulosidic acid (DAA) significantly inhibited superoxide production in advanced glycation end products (AGEs) induced TM3, TM4 and LC-540 cell models. In animal experiment, the model diabetic rat was induced by nicotinamide (230 mg/kg) and streptozotocin (65 mg/kg). Noni juice was tested in three doses (1.24, 2.48 and 66.2 mg/kg, p.o daily) for 4 week. The results indicate that any dose of Noni juice administration for 4 weeks can not improve the hyperglycemia and insulin resistance, but LH and testosterone level were be recovered. The supplementation of Noni juice could significantly restore sperm numbers, motivity, increase activity of anti-oxidative enzyme, decrease lipid peroxidation. The receptor for AGEs expression also deacreased by the Noni jioce administrate on diabetes rats. In conclusion, noni juice might not have the capability to improve the STZ-NA induced hyperglycemia, insulin and leptin resistance. But, not only the antioxidant and antiinflammatory property but also steroidogenesis effect of Noni juice might have contributed for its ability to protect male reproduction.
壹、 緒論 1
貳、 文獻回顧 2
一、 男性生殖與不孕症 2
二、 糖尿病與男性生殖 6
三、 諾麗果 9
參、 實驗設計 14
一、 細胞實驗 14
二、 動物實驗 15
三、 犧牲後分析項目 16
肆、 實驗材料與方法 17
一、 材料與藥品 17
二、 實驗方法 21
伍、 結果與討論 35
一. 以體外實驗探討 AA 及 DAA 抑制 AGEs 誘導 TM3、TM4、LC-540 細胞株之氧化壓力功能 35
二、 以體內實驗探討諾麗果汁對於 STZ-NA 誘導糖尿病雄性大白鼠之生殖功能 38
陸、 結論 46
柒、 參考文獻 47
捌、 圖表 62

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