臺灣博碩士論文加值系統

細胞激素IL-4在免疫反應的調控及分化上扮演了舉足輕重的角色，其中IL-4最為人熟知的功用在於1) 其可引導活化中的T細胞分化成可分泌第二型細胞激素之effector 細胞及 2) 做為B細胞的生長激素之功能。儘管 IL-4有這許多為人熟知的作用，但關於IL-4作用在resting T細胞的功用仍有待瞭解。在這篇論文中，我們發現以IL-4培養過的resting CD8+ T細胞具有1) 細胞表面CD8分子的表現增加及2) 在面對無論是mitogen或是抗原的刺激時，這些細胞皆能有較高的proliferation及IL-2 production。然而，以IL-4培養對於CD4+ T細胞卻有相反的影響。以IL-4培養resting CD4+ T細胞，不但會降低其細胞表面的CD4分子表現，對於活化的刺激更有降低proliferation及IL-2 production的反應。此外，利用Stat6-null老鼠的T細胞，我們發現上述這種IL-4降低CD4+ T細胞CD4表現及活化反應是需要有Stat6 分子的作用，但缺乏Stat6的存在卻不會影響到IL-4培養所達成增強CD8+ T細胞功能。
歸納上述結果，我們報告IL-4培養會對CD4+及CD8+ T細胞造成不同且相反的作用，而這說明了同樣的IL-4R卻能在不同的T細胞傳導執行功能上產生完全相反的作用。

IL-4 is a pleiotropic cytokine which plays an important role in the immune system. The best known function of IL-4 are its ability to 1) drive antigen-activated T cells into Th2 (Tc2)-cytokine secreting effector cells and 2) serve as B cell growth factors. The effect of IL-4 on resting T cells which are known to express IL-4R has not been extensively studied. We report here that IL-4 pretreatment of resting CD8+ T cells 1) upregulated CD8 expression and 2) enhanced antigen and mitogen-induced proliferation and IL-2 production responses. On the other hand, IL-4 pretreatment of resting CD4+ T cells 1) mediated enhancement of immune functions of CD8+ T cells. Taken together, IL-4 pretreatment of resting CD4+ T cells versus CD8+ T cells resulted in differential and opposite effects and suggests that the same IL-4R can mediate functionally distinct/opposite effects on different lineage of downregulated CD4 expression and 2) decreased proliferation and IL-2 production responses. While functional Stat6 molecule was required for IL-4-mediated downregulation of CD4 expression and function by CD4+ T cells, its absence had no effect on IL-4-T cells.

Abbreviation I
Content Table II
Abstract III
Chinese Abstract (中文摘要) IV
Section I Introduction 1
Section IIMaterials and methods
1.Mice 6
2.Isolation of spleen CD4+/CD8+ T cells by
adherence to antibody-coated tissue culture plate 7
3. Analysis of cell surface marker by flow cytometry 9
4.Preparation of CD40L-transfected L cell-
activated B cell blasts as APC 10
5.IL-4 pretreatment of T cells 11
6.T cell activation 11
7.IL-2 bioassay 14
Section IIIResults 16
Section IVDiscussion 24
Section VFigures 32
Section VIReferences 43

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