臺灣博碩士論文加值系統

近年來化粧品產業盛行，眾多廠商不斷開發新穎的商品吸引消費者購買，而面膜不論是在開架店面、及專櫃無所不在，面膜可快速補充肌膚缺乏的水分，使肌膚維持長時間的水潤及滋養。
近期水凝膠興起，水凝膠為三維網狀結構的親水性高分子聚合物，能夠吸水澎潤而不被分解且高強度及高延展性，大多應用於生醫材料、傷口敷料中，是當前相當具有潛力的材料。
因此本研究將台灣在地穀物與水凝膠兩者合併，開發出多功能性水凝膠面膜產品。

With the ever-changing nature of technology, cosmetic has gained popularity. Many manufacturers have to constantly develop the diversified latest products to attract buying. The mask is ubiquitous in physical outlets and counters. The mask can quickly supplementary moisture, leaving the skin moisturized and nourished for a long time. Hydrogels have been gaining popularity recently.
A hydrogel is a three-dimensional (3D) network of hydrophilic polymers that can swell in water and hold a large amount of water without being biodegradable and have high strength and high ductility and malleability. The hydrogel technologies can apply to hygienic products, agriculture, drug delivery systems, food additives, pharmaceuticals, biomedical applications, and wound dressing.
Therefore, this study we combined the hydrogels and locally grown grains to development of multifunctional hydrogel mask.

摘要 Ⅰ
Abstract Ⅱ
致謝 Ⅲ
目錄 Ⅳ
表目錄 Ⅷ
圖目錄 Ⅺ
縮寫名詞對照表 ⅩⅦ
第一章 緒論 1
1.1 研究動機及目的 1
1.2 實驗架構 2
第二章 文獻回顧 3
2.1 微生物與化粧品簡介 3
2.2 水凝膠 4
第三章 材料與方法 6
3.1 實驗材料 6
3.2 實驗儀器與藥品 6
3.2.1 實驗儀器 6
3.2.2 實驗藥品 9
3.3 微生物菌株的培養與保存 12
3.3.1 實驗菌株的保存 12
3.3.2 實驗菌株活化 13
3.3.3 實驗菌株定量 14
3.4 培養基與磷酸鹽緩衝溶液製備方法 14
3.4.1 TSA的製備 14
3.4.2 TSB的製備 15
3.4.3 NA與NB的製備 15
3.4.4 YMA與YMB的製備 16
3.4.5 MEA與MEB的製備 16
3.4.6 RCA與RCB的製備 17
3.4.7 PBS的製備 18
3.5 物性試驗 18
3.5.1 溫度虐待試驗 18
3.5.2 結構虐待試驗19
3.5.3 拉力試驗 19
3.6 抗菌材料及抗菌試驗 20
3.6.1 抗菌材料配置 20
3.6.2 紙錠擴散試驗 20
3.6.3 最小抑菌濃度試驗 21
3.7 面膜基材製備及毒性試驗 22
3.7.1 水凝膠面膜之UV試驗 23
3.7.2 水凝膠面膜之細胞毒性試驗 24
3.8 水凝膠之抗菌試驗 25
3.8.1 面膜基材抗菌試驗 25
3.8.2 產品之防腐試驗 26
3.9 萃取物之製備及樣品 27
3.9.1 萃取物之樣品 28
3.10 萃取物之功效性試驗 29
3.10.1 穀物萃取物對纖維母細胞毒性影響評估 29
3.10.2 穀物萃取物對於抗發炎反應評估 30
3.10.3 抗氧化試驗 31
3.10.3.1 DPPH 自由基清除能力評估 31
3.10.3.2 ABTS 抗氧化能力評估 32
3.10.4 NFα誘發CCD-966SK 纖維母細胞表現MMP-1 33
3.10.5 B16 melanoma cell黑色素表現評估 34
3.10.6 UV試驗測定 35
3.10.7 萃取物之紙錠擴散試驗 36
第四章 實驗結果與討論 37
第五章 結論 123
第六章 參考文獻 126

楊昭順、吳文童、徐瑞蓮、鄭智交、孫豫蘋、陳振興(2008)。化妝品微生物學(第一版)。台中市 : 華格那企業。
行政院衛生署(2005)。化粧品衛生管理條，化粧品衛生管理條例暨有關法規。台北：行政院衛生署。頁7-8。
N. A. Peppas, P. Bures, W. Leobandung, H. Ichickawa. (2000). Hydrogels in Pharmaceutical Formulations. Designed Monomers and Polymers, 50, 27-46.
J. M. Rosiak, F. Yoshii. (1999). Hydrogels and their medical applications. Nuclear Instrument and Methods in Physics research B. 151, 56-64.
Majmudar A. (2012). Multipurpose hydrogel compositions and products, U.S. Patent No. 8, 268, 345B2.
K. Pal, A. K. Banthia, D. K. Majumdar Des Monomers. (2009). Polymeric Hydrogels: Characterization and Biomedical Applications. Designed Monomers and Polymers . 12, 197-220.
L. Klouda, A. G. Mikos (2008). Polymeric hydrogels: Characterization and biomedical applications. Eur J Pharm Biopharm. 68, 34-45.
N. A. Peppas. (1987). Hydrogels in medicine and pharmacy: properties and applications. Vol. 3. CRC Press Inc., 1-208.
R. Barbucci. (2009). Hydrogels: biological properties and applications. Springer- Verlag Italia, Milan, 1-179.
P. J. M. Bouten, M. Zonjee, J. Bender, S. T. K. Yauw, H. van Goor, J. C. M. van Hest, et al. (2014). The chemistry of tissue adhesive materials. Progress in Polymer Science. 39, 1375-1405.
D. B. Stein. (2009). Handbook of hydrogels - properties, preparation＆applications. Nova Science Publishers, Inc., New York.
S. Rimmer. (2011). Biomedical hydrogels- biochemistry, manufacture and medical application, Woodhead Publishing.
K. R. Kamath, K. Park (1993). Biodegradable hydrogels in drug delivery. Advanced Drug Delivery Review. Vol. 11, 59-84.
Rosiak J, Ruciska-Rybus A, Pekala W. (1980). US Patent 4,871,490.
P. A. Janmey, J. P. Winer, J. W. Weisel. (2006). Fibrin gels and their clinical and bioengineering applications. J. R. Soc. Interface. 6(30):1-10. Doi: 10.1098/rsif. 2008. 0327.
Enrica Cal′o, Vitaliy V. Khutoryanskiy. (2015). Biomedical applications of hydrogels: A review of patents and commercial products. European Polymer Journal. 65, 252-267.
Morteza Bahram, Naimeh Mohseni, Mehdi Moghtader. (2015). A introduction to Hydrogels and some recent applications. Doi: 10.5772/64301.
林鴻儒(2018)，「神奇的水膠」，科學發展，第542期，66-72頁。
T. R. Hoare, D. S. kohane. (2008). Hydrogels in drug delivery: Progress and challenges. Polymer. 1993-2007.
A. Vashist, A.Vashist, Y.K. Gupta, S. Ahmad. (2014). Recent advances in hydrogel based drug delivery systems for the human body. Journal of Materials Chemistry B. 2, 147.
C. Elvira, J. F. Mano, J. San Román, R. L. Reis. (2002). Starch-based biodegradable hydrogels with potential biomedical applications as drug delivery systems. Biomaterials. 23, 1955-1966.
A. K. A. Silva, C. Richard, M. Bessodes, D. Scherman, O. W. Merten. (2009). Growth Factor Delivery Approaches in Hydrogels. Biomacromolecules. 10 (1) : 9-18. DOI: 10.1021/ bm801103c.
Muhammad Faheem Akhtar, Nazar Muhammad Ranjha. (2016). Methods of synthesis of hydrogels … A review. Saudi Pharmaceutical Journal. 24, 554-559.
J. A. Hunt, R. Chen, T. van Veen, N. Bryan. (2014). Hydrogels for tissue engineering and regenerative medicine. J. Mater Chem B. 2, 5319-5338.
Jabbari E, Yaszemski MJ, Currier BL. (2006). EP 1 664 168 B1.
Zhao Wen, Jin Xing, Cong Yang, Liu Yuying, Fu Jun. (2013). Degrable natural polymer hydrogels for articular cartilage tissue engineering. J Chem Technol Biotechnol. 88(3), 327-339.
Enas M. ahmed. (2015). Hydrogel: Preparation, characterization, and applications: A review. Journal of Advanced Research. 6, 105-121.
Sina Burkert, Thomas Schmidt, Uwe Gohs, Helmut Dorschner. (2007). Karl-Friedrich cross-linking of poly(N-vinyl pyrrolidone) films by electron beam irradiation. Radiat Phys Chem. 76 (8-9), 1324-1328.
Hacker MC, Mikos AG. (2011). Synthetic polymers, principles of regenerative medicine, 2nd ed,; 587-622.
李俊賢研究助理、孫世勝助研究員。<奈米級有機軟性凝膠材料簡介>。《中央研究院週報》。第1172期。
Andrews, JM. (2001) Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy , 48 (1) :5-16.
Mosmann T. (1983) . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J.Immunol.Methods 65: 55-63.
P.-J. Tsai, Y.-S. Chen, C.-H. Sheu, and C.-Y. Chen. (2011). “Effect of nanogrinding on the pigment and bioactivity of djulis (Chenopodium formosanum Koidz.).” Journal of Agricultural and Food Chemistry. vol. 59, no. 5, 1814–1820,.
C.-C. Chyau, C.-C. Chu, S.-Y. Chen, and P.-D. Duh. (2015). “Djulis (Chenopodiun formosaneum) and its bioactive compounds protect against oxidative stress in human HepG2 cells,” Journal of Functional Foods, vol. 18, pp. 159–170,.