臺灣博碩士論文加值系統

咸豐草學名Bidens pilosa L. ，又名三葉鬼針草、金盞銀盤、南風草等，屬於菊科 (Compositae)植物，一年生草本，高30~100cm。廣分佈於台灣、中國南方、朝鮮、日本、海南島、馬來西亞、印度等熱帶地區。本植物可疏表清熱，解毒，散瘀。治流感，咽喉腫痛，腸炎，痢疾，黃疸，小兒驚風等。全株植物乾燥,以粉碎機粉碎之後，秤重後約1.5公斤,加入甲醇4公升,浸泡一星期,再換新鮮甲醇浸泡一星期,如此方法共浸泡三星期,經過濾殘渣後,得墨綠色甲醇溶液共約12公升,加入活性碳脫色至淺綠色,再過濾去除活性炭,然後減壓濃縮去溶劑,得到黑色濃稠狀抽出物,將抽出物以300ml蒸餾水及300mlx5次 dimethyl ether 萃取,收集的dimethyl ether溶液脫水,並減壓濃縮後得6.5g的濃縮物,以矽膠管柱層析法分離,先依序以hexane,dimethyl ether , acetone , methanol 2000 ml 沖提 ,而粗分為四個fractions,再以矽膠管柱色層分析法、薄層色層分析、再結晶等方法，進行單離、純化。得到四個化合物﹐以IR﹑MASS﹑1H-NMR﹑13C-NMR等光譜鑑定﹐確認結構為:phenylhepta-1,3,5-triyne (I)﹑friedelin (II)﹑alpha-amyrin (III)﹑stigmasterol (IV)。其中friedelin及 alpha-amyrin 為首次於此植物中被分離出來之化合物。

The methanolic extract of the whole plant of bidens pilosa were concentrated and the diethylether-soluble fraction were subjected to systematic separationand purification by column chromatography, thin layer chromatography and recrystallization. Four compounds were isolated and identified by spectral analyses and comparison of their data ( IR, MS, NMR ) as phenylhepta-1,3,5-triyne (I) , friedelin (II) , alpha-amyrin (III) and stigmasterol (IV).