臺灣博碩士論文加值系統

中文摘要

牛磺酸（taurine）為人體、動物體肝臟形成膽酸鹽成分之一，亦是貓的必需胺基酸和海產魚貝類的主要游離胺基酸， 已知其具有保肝、降毒、調節滲透壓、降低血脂質、防止脂質過氧化、抑制神經衝動及調節各組織中之鈣離子激動狀態等功用，為知其是否具有降解慢性肝炎的效果，本研究乃以牛磺酸針對肝功能異常之B或C型病毒性肝炎患者進行試驗，以了解其血液特性（WBC、RBC、Hb、platelet）、血清生化指標（ALT、AST 、cholesterol、TG ）及抗氧化指標（血清中氧化產物TBARS）改變情形。試驗即以24位B或C型病毒性肝炎患者且其ALT（alanine transferase ）或AST（aspartate transferase）值大於正常人數值 2 – 5倍（80 – 200 U/L）進行研究，首先將其分成兩組（各12人），一組為實驗組（牛磺酸組），一組為對照組（控制組）。實驗組給予牛磺酸每日6 g，分三餐飯後食用（即每餐飯後食用2 g）並持續服用三個月，使用前抽血檢查乙次，食用後每月抽血檢查乙次，停用後一個月再抽血檢查乙次，對照組則不給予牛磺酸，每個月抽血檢查乙次，連續四個月。經試驗結果發現，在血液檢查方面，實驗組及對照組皆無變化。在生化檢查部份，實驗組AST、cholesterol和triglyceride在試驗期間雖呈現微幅下降趨勢，但尚未達顯著差異（P＞0.05），對照組則無變化（P＞0.05），但實驗組之ALT值則在服用2和3個月後皆呈現顯著之下降效果（P＜0.05）。在抗氧化（TBARS）方面，實驗組在試驗期間不僅呈現下降趨勢並具有顯著的差異性（P＜0.05），而對照組則亦無變化。因此推論牛磺酸對慢性病毒性肝炎患者，其膳食效果確實具有抗氧化的生理功能，且對肝功能具有部分改善效果，而且一旦停用牛磺酸後，牛磺酸之抗氧化作用仍維持有效。

Abstract

Taurine is an essential amino acid for cats, and found in the tissues of most animal species. Its physiological functions include bile acid conjugation, detoxification, osmoregulation, antioxidation, preventing lipid peroxidation, cell membrane stabilization, neuromodulation and calcium flux regulator, etc. So, we prompted to study the effect of taurine on the liver function of hepatitis B or C patients.
In experiment, the 24 patients with hepatitis B or C and 2 to 5 times over normal activities of alanine transaminase (ALT) or aspartate transaminase (AST) were selected and divided into two groups, taurine group and control group. Each group had 12 patients. In taurine group, patients took 6 g taurine each day divided into 3 times , for three months, and then stop treatment for 1 month. The daily intake（6 g taurine） was divided into three parts and given to patients of taurine group after meal. In control group, patients took placebo without taurine. The blood samples of all patients were collected and analyzed for the biological characters – white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), platelet, ALT, AST, cholesterol, triglyceride (TG) and thiobarbituric acid reactive substances (TBARS) values.
It was found that the blood characters (WBC, RBC, Hb, Platelet) were not significantly different (p>0.05). However, the biochemical indicators （ALT, AST, cholesterol, TG values）of blood serum in the taurine group were all decreased, Among them, ALT showed significantly different (p<0.05) after taking taurine for 2 and 3 months. The levels of TBARS in the serum of taurine group were significantly decreased (p<0.05), but the control group was not.
According to the results of experiment, it indicated that taurine plays an important role in the properties of antioxidation, and has some improvements on the liver function of hepatitis B or C patients. Furthermore, antioxidation effect of taurine was still found on the chronic hepatitis patients once they stop taking dietary taurine.

目錄
目錄………………………………………………………I
表目錄…………………………………………………….IV
中文摘要……………………………………………………...1
英 文 摘 要……………………………………………………...3
壹、 研究背景…………………………………………………………5
貳、文獻整理……………………………………………………………..7
一、牛磺酸（taurine, Tau）………………………………………………7
（一） 牛磺酸之化學性質……………………………………………….7
（二）牛磺酸之生合成與來源…………………………………………..7
（三） 牛磺酸之生理功能………………………………………………8
（四）牛磺酸與肝臟的關係……………………………………………..9
二、肝臟………………………………………………………………….10
（一） 肝臟的構造與功能……………………………………………..10
（二）病毒性肝炎………………………………………………………12
（三）肝功能指標……………………………………………………….18
參、 研究內容………………………………………………………..21
一、前言…………………………………………………………………21
二、實驗材料與方法 22
（一）實驗對象 22
（二）實驗材料與設備 22
（三）實驗方法 24
三、統計分析 29
肆、 結果……………………………………………………………..30
一、血液特性變化 30
1.牛磺酸組 30
2. 對照組 32
二、血液中生化學特性變化 33
1.牛磺酸組 33
2.對照組 36
三、患者血清脂質過氧化物值的變化（TBARS值） 38
1.牛磺酸組 38
2.對照組 38
伍、討論…………………………………………………………………..39
一、試驗者（肝炎患者）基本特性 39
二、患者之血液特性 39
三、患者血液中之生化學特性………………………………………….40
四、患者血清脂質過氧化物值（TBARS值）………………………..41
陸、結論…………………………………………………………………42
柒、參考文獻……………………………………………………………43
附 錄………………………………………………………………..77

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