臺灣博碩士論文加值系統

牛樟芝（Antrodia camphorata）為一新品種且珍貴之食藥用菌菇類，在牛樟樹內壁生長緩慢，具有解毒、抗癌等功效之昂貴中藥材。本研究以探討改變搖瓶培養基單一因子及不同醱酵培養天數對牛樟芝菌絲體生長速率及其成分與代謝產物成分的影響。並著眼於牛樟芝菌絲體成分isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrole-
2,5-dione (C1)之單離純化且以C1為指標成分開發HPLC分析方法，探討牛樟芝菌絲體及代謝產物品質。結果顯示，搖瓶培養以pH 5.0之酵母抽出物作為氮源及果糖作為碳源時，可在第20天時分別得到菌絲體產量為0.434 g/100ml, 0.544 g/100ml及0.944 g/100ml，搖瓶培養以麥芽抽出物2%，蔗糖2%，蛋白腖0.1%，pH 6.0培養菌絲體C1之含量最高；代謝產物中則以培養於酵母抽出物2%，葡萄糖2%，蛋白腖0.1%，pH 5.0培養之濾液中C1含量為最多；此外，菌絲體培養在生物反應器中，培養5天的菌絲體C1含量最高，而培養液內的代謝物則以培養14天時，具有最高C1的產量。
關鍵字：牛樟芝、生物反應器、菌絲體、代謝物

Antrodia camphorata, identified as a new strain, is a valuable medicinal fungus, which grows extremely slowly on the inner wall of Cinnamomum kanehirai Hay. It has the desirable detoxification and anti-cancer properties; hence, it is sold as an expensive Chinese herbal medicine. The objective of this study was to determine the effects of modification of a single element in the cultural medium and different durations of fermentation culture on the growth rates, components, and metabolites of A. camphorata and its mycelia. This study also focused on the isolation, identification and purification of isobutyl-4-[4-(3-methyl-2-butenyloxy) phenyl]-1H-pyrrole-2,5-dione (C1) from A. camphorata mycelia and the development of HPLC analytical method using C1 as a marker to determine the quality of A. camphorata mycelia and their metabolites. Results of the study showed that culturing in shaking flasks at pH 5.0 for 20 days, the total mycelium weight produced was 0.434 g/100 ml, and 0.544 g/100 ml in a medium using yeast extracts as nitrogen source, and 0.944 g/100 ml in a medium using fructose as nitrogen source. The C1 content was the highest when mycelia were cultured in shaking flasks with a medium containing 2 % yeast extracts, 2% fructose, and 0.1 % peptone at pH 6.0. However, the C1 content was the highest in filtered solution of the metabolites of mycelia cultured in a medium containing 2% yeast extracts, 2% glucose, and 0.1% peptone at pH 5.0. In addition, the C1 content was the highest when mycelia were cultured in a bioreactor for 5 days and also the highest in the metabolites when mycelia were cultured in a medium solution for 14 days.
Keywords: Antrodia camphorata, bioreactor, mycelium, metabolite

目錄
中文摘要…………………………………………………………………Ⅰ
英文摘要……………………………………………………………………Ⅲ
誌謝…………………………………………………………………………Ⅴ
目錄…………………………………………………………………………Ⅷ
表次索引………………………………………………………………… Ⅹ
圖次索引……………………………………………………………… ⅩⅡ
壹、前言…………………………………………………………………… 1
貳、文獻回顧……………………………………………………………….4
參、實驗材料與方法………………………………………………………20
一、試驗材料…………………………………………………………20
二、試驗方法…………………………………………………………25
肆、結果……………………………………………………………………32
一、樟芝菌絲體培養條件試驗………………………………………32
二、樟芝菌絲體及其醱酵培養液成分分析…………………………44伍、討論……………………………………………………………………62
一、牛樟芝菌絲體培養………………………………………………62
二、牛樟芝菌絲體及其過濾液成分定量分析試驗…………………64
三、樟芝菌絲體醱酵槽培養不同天數之成分定量分析……………67
參考文獻……………………………………………………………………68
附錄一　菇類的食品機能…………………………………………………73
附錄二　……………………………………………………………………74
附錄三　牛樟芝子實體成分………………………………………………75
附錄四　牛樟芝菌絲體成分………………………………………………77
附錄五　MEA培養基成分……………………………………………………78
附錄六　……………………………………………………………………79
作者簡介……………………………………………………………………80

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