臺灣博碩士論文加值系統

黃耆為中醫廣泛使用的藥材，具有免疫調節、抗氧化等功效，本研究目的為探討黃耆對提升運動表現及延緩衰老之功效性。實驗分兩部分，實驗一以雄性4月齡SAMP8 (senescence-accelerated mouse prone-8；SAMP8)小鼠為實驗對象，分為對照組及低(410mg/ kg BW /day)、中(1230mg/ kg BW /day)、高劑量試驗組(4100mg/ kg BW /day)，對照組給予ddH2O，連續管灌餵食8週。結果顯示，補充黃耆組皆能降低運動測試中第2、4及6週之電擊次數(p<0.05)；在游泳力竭時間上高劑量組之力竭時間明顯較對照組長 (p<0.05)。在血液分析結果顯示中、高劑量組之血尿素氮及血乳酸濃度顯著低於對照組(p<0.05)，其餘生化值皆無差異。
實驗二選用3月齡SAMP8雄性及雌性小鼠，隨機分成對照組、實驗組A (黃耆原料，820mg /kg BW/day)及實驗組B(黃耆複方，6.2 ml/kg BW/day)，實驗為期8週。結果顯示，實驗組皆具較佳之學習記憶能力 (p<0.05)。老化指數部分，實驗B組小鼠老化指數總分顯著低於對照組 (p<0.05)。腦部及肝臟組織老化之生物活性指標結果發現，實驗組皆能顯著降低腦部及肝臟TBARS含量(p<0.05)。在抗氧化分析結果中，實驗組之SOD(superoxide dismutase)、Catalase活性均顯著高於對照組(p<0.05)，其中又以實驗B組的效果較佳。
綜合以上兩實驗結果，小鼠補充黃耆可有效地提升運動能力表現，增加小鼠體內抗氧化能力，減少腦部及肝臟之氧化損傷，進而達到抗疲勞及延緩衰老之效果。

Astragalus is a widely used medical material in Chinese medicine, which has immunosmodulatory and antioxidant effects. The purpose of this study was to investigate the effects of Astragalus on improving sports performance and anti-aging senescence accelerated mice(SAMP8). The experiment divided to two parts, the first experiment was used 4-month-old male SAMP8 mice. The forty mice were randomly divided into control group, low dose group (410 mg / kg BW / day), medium dose group (1230 mg / kg BW / day) and high dose group (4100 mg / kg BW / day). In the exercise test, the retention frequencies of 2nd, 4th and 6th week of experimental groups were significantly lower than control group (p<0.05). The swimming exhaustion time of the experimental groups were significantly longer than control group (p<0.05). Analysis of blood lactic acid and blood urea nitrogen concentration showed that medium dose and high dose groups were significantly lower than control group ( p<0.05).
In the second experiment, 3-month-old male and female SAMP8 mice were divided into three groups, including control group, groupA (astragalus, 820mg/kgBW/day) and group B (astragaluscomplex, 6.2ml/kgBW/day). The experimental period lasted for 8 weeks. The results showed that there was no significant difference in food intakes, water consumption and body weight among groups. The single-trial passive avoidance test and active shuttle avoidance test results showed that the learning and memory ability of the group A and group B were better than control group (p<0.05). The total aging score of group B was significantly lower than other groups (p<0.05). Analysis of tissue biochemical indicators showed that group A and group B could decrease TBARS contents in brain and liver (p<0.05). The analysis of antioxidant capacity, the experimental groups increased activity level of superoxide dismutase (SOD) and catalase (p<0.05).
In summary, the supplement of astragalus could improve sports performance, increase the antioxidant capacity, reduce oxidative damage and then delaying aging.

摘要I
目錄V
表目錄IX
圖目錄XII
第一章 前言1
第二章 文獻回顧5
第一節 黃耆5
一、黃耆簡介5
二、黃耆選材與分類7
三、黃耆的生理功能10
四、黃耆多醣與黃耆皂苷之功效12
第二節 運動與疲勞13
第三節 老化17
一、老化現象17
二、老化與自由基20
第四節 老化促進小鼠25
一、老化促進小鼠品系25
二、老化促進小鼠之特徵25
三、老化指數評估28
第三章 材料與方法31
【實驗一】
第一節 實驗動物31
第二節 實驗材料與劑量32
一、實驗材料與動物分組32
二、實驗劑量換算32
第三節 實驗流程與方法33
一、實驗流程33
二、運動測試35
三、前肢抓力測定36
四、游泳力竭測試37
五、血清生化分析38
六、肝臟肝醣分析38
第四節 統計分析38
【實驗二】
第一節 實驗動物39
第二節 實驗材料及劑量39
一、實驗材料與動物分組39
二、實驗劑量換算40
第三節 實驗流程與方法40
一、實驗流程40
二、活動量(LOCOMOTION)測試42
三、老化指數(GRADING SCORE)評估43
四、血清生化值分析44
五、腦部與肝臟組織老化之生物活性指標測定45
第四節 統計分析54
第四章 實驗結果55
【實驗一】
第一節 體重、攝食量、飲水量55
第二節 運動表現能力56
一、運動測試56
二、前肢抓力與游泳力竭測試68
第三節 器官重量之變化72
第四節 血清生化值分析74
第五節 血乳酸及肝醣含量76
【實驗二】
第一節 體重、攝食量、飲水量80
第二節 老化指數81
第三節 活動量83
第四節 器官重量之變化84
第五節 血清生化值分析86
第六節 腦部與肝臟組織老化之生物活性指標測定88
一、腦部組織之生物活性指標測定88
二、肝臟組織老化之生物活性指標測定100
三、肝臟抗氧化生化指標測定103
第五章 綜合討論109
第六章 結論與建議112
第七章 參考文獻114

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