臺灣博碩士論文加值系統

在眾多的營養增補劑當中，Co Q10 (Coenzyme Q10 , ubiquinone) 已逐漸成為研究的焦點。Co Q10為一內生性的脂溶性物質，且被認為有捕捉自由基及抑制脂質過氧化的作用。雖然Co Q10可以由體內自行合成，但在運動後血中Co Q10的濃度會下降，此可能因肌肉中Co Q10的轉換率提升以及高代謝壓力下，脂質過氧化物大量產生所致。本研究目的在探討Co Q10的補充是否能抑制運動誘發的脂質過氧化及肌肉之損傷，並進一步探討Co Q10的補充對運動表現的影響。實驗以16名女性足球員為對象，分為Co Q10組 (120 mg·d-1) 及安慰劑組，進行為期4週的補充。對耐力運動員進行兩階段的運動負荷測試:先於65% VO2max的運動負荷下進行運動測試60分鐘，之後於90% VO2max負荷跑至力竭為止。結果顯示Co Q10補充可抑制運動後脂質過氧化物MDA濃度的上升，並有降低肌肉損傷指標Creatine Kinase 的趨勢，但與安慰劑組間未達顯著差異。Co Q10可減省運動時血中vitamin E的損耗，但對於血漿總穀胱甘月太之濃度並無影響。Co Q10的補充對生理代謝物質如血中游離脂肪酸、乳酸及血糖的濃度並無影響。另外Co Q10的補充亦不會增進有氧運動表現的指標，如最大心跳率 (HRmax)、最大攝氧量(VO2max) 、呼吸交換率(RR)以及運動至力竭的時間。故補充Co Q10可能會抑制運動中脂質過氧化物的形成，但對耐力運動表現可能則無所增益。

Among many ergogenic aids, Coenzyme Q10 (Co Q10) has earned much attention in the research field of oxidative stress. Co Q10, an endogenous lipid-soluble compound, can scavenge free radicals and attenuate lipid peroxidation. Co Q10 level in the blood has been demonstrated decrease in athletes after intense training, and it is suggested that such low plasma levels of Co Q10 after exercise may indicate the increase of intracellular metabolic turnover of Co Q10 and peroxidative damage. The purpose of this study is to examine whether the supplementation of Co Q10 can decrease lipid peroxides formation and muscular damage in athletes. Sixteen female football players were randomly assigned into Co Q10 and placebo group. Subjects either ingested 120 mg Co Q10 or placebo for 4 weeks. Subjects exercised at 65% VO2max for 60 m'''''''''''''''', and followed by 90% VO2max until exhaustion. Although there was no significant difference in malondialdehyde and creatine kinase values for two groups, the results showed that Co Q10 supplementation can suppress the increase of lipid peroxides after exercise, and has a trend to decrease creatine kinase for Q10 group. Co Q10 can lower the depletion of α-tocopherol during exercise , but had no effect on total glutathine concentration. Supplementation of Co Q10 has no effect on physiological metabolites such as serum free fatty acid ﹑lactate and glucose. Besides, Co Q10 can’t enhance aerobic exercise indexes such as maximal heart rate﹑maximal oxygen consumption﹑respiratory exchange rate and the exhaustion time. In conclusion, Co Q10 may suppress peroxides formation during exercise, but may had no benefit on exercise performance.

目錄
頁次
中文摘要………………………………………………………….I
英文摘要………………………………………………………….II
誌謝……………………………………………………………….III
目錄……………………………………………………………….V
表目錄…………………………………………………………….X
圖目錄…………………………………………………………….XI
簡寫表…………………………………………………………….XII
第一章 緒論
第一節 研究動機………………………………………………….1
第二節 研究目的………………………………………………….4
第三節 研究假設………………………………………………….4
第二章 文獻回顧
第一節耐力運動能量代謝……………………….……………4
一、運動之供能統…………………………………………….6
二、耐力運動之限制因子……………………………………..7
(一) 肌醣、肝醣耗竭及低醣………………………………7
(二) 乳酸堆積……………………………………………....7
(三) 中樞神經疲勞………………………………………...8
第二節 Co Q10 的簡介…………………………………………9
一、對心臟病患的療效…………………………………...10
二、心血管系統方面……………………………………..10
三、改善慢性疲勞徵候…………………………………..11
四、提昇有氧代謝能力…………………………………..11
五、具抗氧化的效力……………………………………..11
六、運動表現的增進………………………………………13
第三節 耐力運動與氧化傷害…………………………………14
一、自由基產生的機制…………………………………….14
二、耐力運動與氧化壓力之研究………………………….17
(一) 脂質過氧化物方面………………………………..17
(二) 抗氧化能力之狀況………………………………..19
(三) 細胞肌肉損傷方面 ………………………………21
第四節 補充抗氧化劑對耐力運動之影響…………………….22
一、補充抗氧化劑對自由基的影響………………………22
二、 補充抗氧化劑對運動表現之影響…………………..24
第三章 材料與方法
第一節研究對象………………………………………………27
第二節 實驗設計………………………………………………27
第三節 實驗處理………………………………………………28
第四節 實驗流程……………………………………………...29
第五節 使用儀器及測量方法…………………………………30
一、體脂肪測量……………………………………………30
二、最大攝氧量(VO2max)測試……………………………..31
三 、65%VO2max耐力運動…………………………………32
四、血液分析………………………………………………33
(一)血液前處理…………………………………………..33
(二) 游離脂肪酸(FFA)……………………………………33
(三)血乳酸(Lactate)…………………………………….…34
(四)血糖濃度的測定……………………………………...34
(五)脂質過氧化物－MDA的測定……………………….35
(六)細胞損傷的評估－(CK)…………………………..….35
(七)血中總麩胱甘月太 ( t-GSH) 的測定…………………..36
(八) vitamin E的測定……………………………………..37
第六節 資料處理………………………………………………..38
第四章 結果………………………………………………………39
第一節基本資料的分怖情形………………………………..39
第二節 飲食中營養素及能量攝取情形………………………39
第三節 Co Q10對漸增性運動心肺功能之影響 ……………..39
第四節 Co Q10對65%VO2max耐力運動下心肺反應、血液生化值及運動表現之影響……………………………………...40
第五章討論………………………………………………………..41
第一節 CoQ10對漸增性運動心肺功能之影響………………...41
一、心跳……………………………………………………41
二、最大攝氧量……………………………………………42
第二節 Co Q10對血液生化值變化之影響……………………43
一、血醣 …………………………………………………...43
二、游離脂肪酸……………………………………………44
三、血乳酸…………………………………………………46
第三節 CoQ10對運動表現之影響……………………………..48
第四節 CoQ10對體內抗氧化效力之影響……………………..48
一、 肌肉損傷指標 ─ 肌酸激酉每 (CK)………………….48
二、穀胱胺月太 (GSH)……………………………………...49
三、脂質過氧化物 ─ 丙二醛 (MDA)………………….50
四、vitamin E ……………………………………………..52
第六章 結論………………………………………………………..61
參考文獻………………………………………………………………63
附錄一、受試者同意書………………………………………………72
附錄二、二十四小時飲食回憶紀錄表………………………………73
附錄三、基本資料表…………………………………………………74
附錄四、運動訓練問卷………………………………………………75
附錄五、受試者之原始資料…………………………………………76

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